| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| A_1.fq.gz | 45,784,482 | 0 | 150-150 | 47 |
| A_2.fq.gz | 45,784,482 | 0 | 150-150 | 47 |
| D_1.fq.gz | 47,700,555 | 0 | 150-150 | 46 |
| D_2.fq.gz | 47,700,555 | 0 | 150-150 | 47 |
| G_1.fq.gz | 48,183,768 | 0 | 150-150 | 47 |
| G_2.fq.gz | 48,183,768 | 0 | 150-150 | 47 |
| L_1.fq.gz | 41,997,745 | 0 | 150-150 | 47 |
| L_2.fq.gz | 41,997,745 | 0 | 150-150 | 47 |
| S2_1.fq.gz | 44,968,804 | 0 | 150-150 | 47 |
| S2_2.fq.gz | 44,968,804 | 0 | 150-150 | 47 |
| S4_1.fq.gz | 43,364,251 | 0 | 150-150 | 47 |
| S4_2.fq.gz | 43,364,251 | 0 | 150-150 | 47 |
| S5_1.fq.gz | 43,177,450 | 0 | 150-150 | 47 |
| S5_2.fq.gz | 43,177,450 | 0 | 150-150 | 48 |
| S8_1.fq.gz | 43,700,910 | 0 | 150-150 | 46 |
| S8_2.fq.gz | 43,700,910 | 0 | 150-150 | 47 |
Library Sizes ranged between 41,997,745 and 48,183,768 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Drerio Transcriptome
GC Content Distributions for all reads showing theoretical GC content from the Drerio Transcriptome
Universal Adapter Content
Total overrepresented sequences for each library
## Overrepresented_sequences missing from A_1.fq.gz## Overrepresented_sequences missing from A_2.fq.gz## Overrepresented_sequences missing from D_1.fq.gz## Overrepresented_sequences missing from D_2.fq.gz## Overrepresented_sequences missing from G_1.fq.gz## Overrepresented_sequences missing from G_2.fq.gz## Overrepresented_sequences missing from L_1.fq.gz## Overrepresented_sequences missing from L_2.fq.gz## Overrepresented_sequences missing from S2_1.fq.gz## Overrepresented_sequences missing from S2_2.fq.gz## Overrepresented_sequences missing from S4_1.fq.gz## Overrepresented_sequences missing from S4_2.fq.gz## Overrepresented_sequences missing from S5_1.fq.gz## Overrepresented_sequences missing from S5_2.fq.gz## Overrepresented_sequences missing from S8_1.fq.gz## Overrepresented_sequences missing from S8_2.fq.gz## No overrepresented sequences were detected by FastQCR version 3.6.1 (2019-07-05)
Platform: x86_64-pc-linux-gnu (64-bit)
locale: LC_CTYPE=en_AU.UTF-8, LC_NUMERIC=C, LC_TIME=en_AU.UTF-8, LC_COLLATE=en_AU.UTF-8, LC_MONETARY=en_AU.UTF-8, LC_MESSAGES=en_AU.UTF-8, LC_PAPER=en_AU.UTF-8, LC_NAME=C, LC_ADDRESS=C, LC_TELEPHONE=C, LC_MEASUREMENT=en_AU.UTF-8 and LC_IDENTIFICATION=C
attached base packages: parallel, stats, graphics, grDevices, utils, datasets, methods and base
other attached packages: readr(v.1.3.1), stringr(v.1.4.0), magrittr(v.1.5), scales(v.1.0.0), pander(v.0.6.3), kableExtra(v.1.1.0), dplyr(v.0.8.3), ngsReports(v.1.1.1), tibble(v.2.1.3), ggplot2(v.3.2.0) and BiocGenerics(v.0.30.0)
loaded via a namespace (and not attached): bitops(v.1.0-6), matrixStats(v.0.54.0), lubridate(v.1.7.4), webshot(v.0.5.1), RColorBrewer(v.1.1-2), httr(v.1.4.0), GenomeInfoDb(v.1.20.0), tools(v.3.6.1), backports(v.1.1.4), R6(v.2.4.0), lazyeval(v.0.2.2), colorspace(v.1.4-1), withr(v.2.1.2), tidyselect(v.0.2.5), compiler(v.3.6.1), rvest(v.0.3.4), Biobase(v.2.44.0), Cairo(v.1.5-10), xml2(v.1.2.0), DelayedArray(v.0.10.0), plotly(v.4.9.0), ggdendro(v.0.1-20), labeling(v.0.3), digest(v.0.6.20), Rsamtools(v.2.0.0), rmarkdown(v.1.14), XVector(v.0.24.0), pkgconfig(v.2.0.2), htmltools(v.0.3.6), highr(v.0.8), htmlwidgets(v.1.3), rlang(v.0.4.0), rstudioapi(v.0.10), shiny(v.1.3.2), zoo(v.1.8-6), hwriter(v.1.3.2), jsonlite(v.1.6), crosstalk(v.1.0.0), BiocParallel(v.1.18.0), RCurl(v.1.95-4.12), GenomeInfoDbData(v.1.2.1), Matrix(v.1.2-17), Rcpp(v.1.0.1), munsell(v.0.5.0), S4Vectors(v.0.22.0), stringi(v.1.4.3), yaml(v.2.2.0), MASS(v.7.3-51.4), SummarizedExperiment(v.1.14.0), zlibbioc(v.1.30.0), plyr(v.1.8.4), grid(v.3.6.1), promises(v.1.0.1), crayon(v.1.3.4), lattice(v.0.20-38), Biostrings(v.2.52.0), hms(v.0.5.0), zeallot(v.0.1.0), knitr(v.1.23), pillar(v.1.4.2), GenomicRanges(v.1.36.0), reshape2(v.1.4.3), stats4(v.3.6.1), glue(v.1.3.1), evaluate(v.0.14), ShortRead(v.1.42.0), latticeExtra(v.0.6-28), data.table(v.1.12.2), vctrs(v.0.2.0), httpuv(v.1.5.1), gtable(v.0.3.0), purrr(v.0.3.2), tidyr(v.0.8.3), assertthat(v.0.2.1), xfun(v.0.8), mime(v.0.7), xtable(v.1.8-4), later(v.0.8.0), viridisLite(v.0.3.0), truncnorm(v.1.0-8), GenomicAlignments(v.1.20.1) and IRanges(v.2.18.1)